[Provirus structure and expression in rat cell lines transformed by the avian sarcoma virus].
Brashishkite Da, Galetskiĭ Sa, P Kulifeĭ, M Urbanchikova, Alena Lizonová
Abstract
Brashishkite Da, Galetskiĭ Sa, P Kulifeĭ, M Urbanchikova, Alena Lizonová
Abstract
Two type of exogenous action on transformed cells have been investigated. The first system includes clones of TWERC cells transformed by Rous sarcoma virus and cultivated in the presence of 5-bromodeoxyuridine. The second system consists of spontaneously transformed rat cells Sam IV superinfected with avian sarcoma virus B-77. In these cell lines and clones the structure of the provirus and its expression have been analyzed by different molecular probes.
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Two type of exogenous action on transformed cells have been investigated. The first system includes clones of TWERC cells transformed by Rous sarcoma virus and cultivated in the presence of 5-bromodeoxyuridine. The second system consists of spontaneously transformed rat cells Sam IV superinfected with avian sarcoma virus B-77. In these cell lines and clones the structure of the provirus and its expression have been analyzed by different molecular probes.
Key concepts: Provirus, Rous sarcoma virus, Virology, Biology, Virus, Retrovirus, Cell culture, Molecular biology