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Characteristics of messenger ribonucleoprotein and putative messenger RNA from Ehrlich ascites tumor cells.

Nakai Gs

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Abstract

Ehrlich ascites tumor cell messenger ribonucleoprotein (mRNP) and putative messenger RNA (mRNA) were isolated under conditions minimizing RNA degradation by ribonucleases and non-specific protein-RNA interaction, mRNP dissociated from polysomes by puromycin and EDTA sedimented to 185S. Putative messenger RNA released by proteinase K sedimented from 50 to 97S, with a maximum molecular weight approximating 6-25 X 10(6) daltons.

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What this paper is about

Ehrlich ascites tumor cell messenger ribonucleoprotein (mRNP) and putative messenger RNA (mRNA) were isolated under conditions minimizing RNA degradation by ribonucleases and non-specific protein-RNA interaction, mRNP dissociated from polysomes by puromycin and EDTA sedimented to 185S. Putative messenger RNA released by proteinase K sedimented from 50 to 97S, with a maximum molecular weight approximating 6-25 X 10(6) daltons.

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Available abstract

Ehrlich ascites tumor cell messenger ribonucleoprotein (mRNP) and putative messenger RNA (mRNA) were isolated under conditions minimizing RNA degradation by ribonucleases and non-specific protein-RNA interaction, mRNP dissociated from polysomes by puromycin and EDTA sedimented to 185S. Putative messenger RNA released by proteinase K sedimented from 50 to 97S, with a maximum molecular weight approximating 6-25 X 10(6) daltons.

Key concepts: Polysome, Messenger RNP, Messenger RNA, Five-prime cap, Ribonucleoprotein, Puromycin, RNA, Mature messenger RNA

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Characteristics of messenger ribonucleoprotein and putative messenger RNA from Ehrlich ascites tumor cells. — Research Paper | ScholarLens