日本血吸虫ONA多价疫苗SjGST-FABP/pcZDNA3的构建及其保护性免疫研究

[Objective] To construct and develop the multivalent DNA vaccine SjGST-FABP/pcDNA3 and observe its protective efficacy against Schistosoma japonicaum in mice. [Methods] According to the SjGST ORF in plasmid pGEX-4T-1 and the cDNA sequence of SjFABP (fatty acid binding protein), two fragements were spliced by recombinant PCR. Fusion fragement was directionaly cloned into a eukaryotic expression vector pcDNA3 to construct the recombinant multivalent DNA vaccine SjGST-FABP/pcDNA3. Identified by restriction analysis and sequence, the recombinant multivalent plasmid was extracted, purified and inoculated into BALB/c mice by intramuscular injection. Mice were challenged with Schistosoma japonicum cercariae percutaneously on abdominal skin then killed. Adult worms and eggs in liver were calculated, respectively. [Results] The recombinant multivalent plasmid S1GST-FABP/ pcDNA3 was confirmed by double endonucleases digestion, PCR and sequencing, indicating its successful construction. Mice vaccinated with recombinant multivalent DNA vaccine SjGST-FABP/ pcDNA3 revealed 42.39% worm reduction rate and 56.09% liver eggs reduction rate per gram (LEPG) (P＜0.05), as compared with the control group. [Conclusion] The multivalent DNA vaccine SjGST-FABP/pcDNA3 which expressed SJGST-FABP was successfully constructed and elicit moderate significant immunopretection against Schistosoma japonicum.