Cutaneous leishmaniasis in Kenya: Sergentomyia garnhami (Diptera Psychodidae), a possible vector of Leishmania major in Kitui District: a new focus of the disease.

Investigations on phlebotomine sandflies in Tseikuru, Kitui District, Kenya were carried out to determine the vectors of Leishmania major. Sandflies were trapped from animal burrows over a period of one year using castor oil-smeared sticky traps. Nineteen phlebotomine sandfly species were identified and their prevalence in this habitat determined. Dissections on the parous sandflies resulted in the isolation of Leishmania parasites from Phlebotomus martini and Sergentomyia garnhami. The isolated parasites were cultured in NNN medium and were consequently mass cultured in RPMI medium for biochemical characterisation. The parasites from S. garnhami were typed as Leishmania major.