[A study on type I, III and IV collagen production in CCl4 induced rat liver fibrosis].

OBJECTIVE: To study the dynamic changes of alpha 1 (I), alpha 1 (III) and alpha I (IV) procollagen mRNA and collagen-producing cells during CCl4 induced SD rat liver fibrogenesis (20 weeks). METHODS: The investigations were performed using Northern blot analysis, in situ hybridization and immunohistochemical techniques. RESULTS: The increased expression of alpha 1 (III) procollagen mRNA during fibrogenesis by Northern blot analysis was the most predominant among the three mRNAs studied. However, the increase of alpha 1 (IV) procollagen mRNA expression occurred earlier, while the expression of alpha 1 (I) mRNA did not increase until the middle stage of the experiment. Desmin positive and/or smooth muscle actin positive Ito cells and myofibroblasts (MFs) in and around the necrotic areas expressed alpha 1 (I), alpha 1 (III) and alpha 1 (IV) procollagen mRNA signals detected by in situ hybridization during the early stage of the experiment. All the three procollagen mRNAs were mostly localized in fibroblasts (Fbs) and MFs in the septa during the mid and late stages of fibrosis. CONCLUSIONS: Fbs and MFs were considered as important Col I, Col III and Col IV producing cells in liver fibrosis. Sinusoid endothelia were involved in Col IV synthesis in the fibrotic liver.