Neuronal Na+, K+-ATPase in the peripheral nerve fibers.
S Dolapchieva, K Ichev, L. Surchev
Abstract
S Dolapchieva, K Ichev, L. Surchev
Abstract
ATPase activity was localized by means of Wachstein-Meisel's method in rat sciatic nerve fibers. Using controls with ouabain, the presence of alpha + (neuronal) Na+, K+-ATPase was examined. The enzyme occurs in the ATPase reaction of the myelin-forming membranes, axoplasm and Schwann cell cytoplasm. Its presence in the Schwann cell plasma membrane is only admittable. The ATPase activity of the compact myelin and axolemma was exclusively of alpha + type of Na+, K+-ATPase.
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ATPase activity was localized by means of Wachstein-Meisel's method in rat sciatic nerve fibers. Using controls with ouabain, the presence of alpha + (neuronal) Na+, K+-ATPase was examined. The enzyme occurs in the ATPase reaction of the myelin-forming membranes, axoplasm and Schwann cell cytoplasm. Its presence in the Schwann cell plasma membrane is only admittable. The ATPase activity of the compact myelin and axolemma was exclusively of alpha + type of Na+, K+-ATPase.
Key concepts: Axolemma, Axoplasm, Schwann cell, ATPase, Ouabain, Myelin, Cytoplasm, Sciatic nerve