2016•Человек и его здоровьеOpen access
Метаболиты оксида азота в тканях, сыворотке крови, мононуклеарных и мезенхимальных стволовых клетках
А.А. Чеботарёва, I. O. Komarevtseva, Р.М.С. Юсуф, Ю. А. Черных, И.А. Вишницкая, Е. В. Комаревцева, D. Posternak, Н.М. Серегина, А.А. Журба, Р.П. Морару-Бурлеску, Р.В. Чередниченко, С.В. Тертычная-Телюк, Т.П. Яковлева, Ю.В. Кравцова, К.А. Симиненко
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Abstract
Nitric oxide radical (NO•) is an important mediator of both physiological and pathophysiological processes. The NO• radical is rapidly metabolized into the stable end-products - nitrite and nitrate. NO x (nitrate and nitrite) is a stable end product of nitric oxide (NO). In most body fluids, including plasma, most of nitrite is converted into nitrate; thus, determination of nitrite alone is meaningless. The most commonly used nitrite assay is based on the Griess diazotization reaction, which is specific for nitrite and does not detect nitrate. Therefore, nitrate in samples must first be reduced to nitrite; subsequent nitrite determination, thus, yields the total nitrite + nitrate concentration of the sample. NO 3 - plus NO 2 - (NO x ) was measured as NO 2 - after enzymatic conversion of NO 3 - to NO 2 - . After the conversion, the spectrophotometric measurement of nitrite is accomplished by using the Griess Reaction. Clearly, the real value of determining NO• or its metabolites - nitrite and nitrate in clinical practice still need to be proved.
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