1978PubMedRequires access

[Distribution of 5'-nucleotidase in fractions of rat yolk-sac after differential centrifugation and isopycnic sucrose gradient centrifugation].

Thorsten Oliver Goetze, W. Hühn

Open publisher page 0 citations

Abstract

The 5'-nucleotidase considered as a marker enzyme of the plasma membranes shows a highly specific acitvity in both nuclear and mitochondrial-lysosomal fractions of the rat yolk-sac. In isopycnic sucrose gradient fractionation of the mitchondrial-lysosomal fraction a relative accumulation of this enzyme could be shown in the density level of the phagolysosomes (d = 1,16--1,20).

About this research paper

What this paper is about

The 5'-nucleotidase considered as a marker enzyme of the plasma membranes shows a highly specific acitvity in both nuclear and mitochondrial-lysosomal fractions of the rat yolk-sac. In isopycnic sucrose gradient fractionation of the mitchondrial-lysosomal fraction a relative accumulation of this enzyme could be shown in the density level of the phagolysosomes (d = 1,16--1,20).

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The 5'-nucleotidase considered as a marker enzyme of the plasma membranes shows a highly specific acitvity in both nuclear and mitochondrial-lysosomal fractions of the rat yolk-sac. In isopycnic sucrose gradient fractionation of the mitchondrial-lysosomal fraction a relative accumulation of this enzyme could be shown in the density level of the phagolysosomes (d = 1,16--1,20).

Key concepts: Isopycnic, Differential centrifugation, Sucrose gradient, 5'-nucleotidase, Chemistry, Centrifugation, Yolk sac, Density gradient

Related papers

Back to paper searchBrowse research topicsOriginal source
[Distribution of 5'-nucleotidase in fractions of rat yolk-sac after differential centrifugation and isopycnic sucrose gradient centrifugation]. — Research Paper | ScholarLens