[Establishment of transgenic mice of hepatitis B virus X gene (adr subtype) mediated by spermatogonial cells].

OBJECTIVE: To test the possibility of using spermatogonial cells as vector in the establishment of HBV X gene transgenic mice. METHODS: Eukaryoti expression vector pcDNA3-X of hepatitis B virus X gene was constructed. The recombinant pcDNA3-X packed by liposome was injected into testicle tissue of male C57BL/6N mice using microvolume injector. Six weeks after the first injection, the mice were crossed with female mice. Polymerase chain reaction and immunohistochemistry were applied to identify the viral DNA(HBx gene) harbored n liver tissue. RESULTS: The sequence of the X gene is consistent with that reported in literature. The X gene can be expressed in Hela cells. The positive rate in daughter mice was 6.25%. CONCLUSION: It is possible to use spermatogonial cells as vector to establish HBx transgenic mice.