1992•PubMedRequires access
Is a trypsin-like protease of mites a Der f III allergen?
Thoru Ando, R Homma, Ino Y, Ito G, Anna Miyahara, Hiroshi Yamakawa, Masahiro Iwaki, Y Okumura, Matsunobu Suko, Michiko Haida
Open publisher page 5 citations
Abstract
A trypsin-like protease was purified from mite (Dermatophagoides farinae) fecal extract. In SDS-PAGE, the mite trypsin-like protease showed a single band at 34 kD. The purified trypsin-like protease possessed potent allergenic activity. Both the twenty N-terminal amino acid sequence and the amino acid composition of the purified protease were very similar to those of Der f III. These data strongly suggest that the trypsin-like protease in the mite is a Der f III allergen.