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2004•Humana Press eBooksRequires access

Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR

Scott Bowden, Kathy Merlock Jackson, Margaret Littlejohn, Stephen Locarnini

Open publisher page 37 citations

Abstract

Hepadnaviruses utilize an unusual replication strategy. On infection, the partially double-stranded open circular genomic DNA is transported to the hepatocyte nucleus, where host-cell enzymes convert it to a relaxed circular fully double-stranded molecule. From this replicative form is generated a covalently closed circular (ccc) DNA, which associates with cellular histones to form a viral minichromosome ( 1 , 2 ). The HBV (hepatitis B virus) ccc DNA remains in the cell nucleus and serves as the transcriptional template for HBV-RNA production. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

About this research paper

What this paper is about

Hepadnaviruses utilize an unusual replication strategy. On infection, the partially double-stranded open circular genomic DNA is transported to the hepatocyte nucleus, where host-cell enzymes convert it to a relaxed circular fully double-stranded molecule. From this replicative form is generated a covalently closed circular (ccc) DNA, which associates with cellular histones to form a viral minichromosome ( 1 , 2 ). The HBV (hepatitis B virus) ccc DNA remains in the cell nucleus and serves as the transcriptional template for HBV-RNA production. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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OpenAlex reports 37 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Hepadnaviruses utilize an unusual replication strategy. On infection, the partially double-stranded open circular genomic DNA is transported to the hepatocyte nucleus, where host-cell enzymes convert it to a relaxed circular fully double-stranded molecule. From this replicative form is generated a covalently closed circular (ccc) DNA, which associates with cellular histones to form a viral minichromosome ( 1 , 2 ). The HBV (hepatitis B virus) ccc DNA remains in the cell nucleus and serves as the transcriptional template for HBV-RNA production. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Key concepts: cccDNA, Minichromosome, Circular DNA, DNA, Hepatitis B virus, Biology, Histone, RNA

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