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Purification of phosphoglycerate kinase and glyceraldehyde phosphate dehydrogenase by DEAE-sepharose fast flow chromatography.

Ting Tan, Zeyang Shen

Abstract

The purification of phosphoglycerate kinase (PGK) and glyceraldehyde phosphate dehydrogenase (GAPDH) from soy bean was studied by a DEAE-sepharose fast flow chromatography. When scaling up, the diameters of columns increase but the heights of columns were maintained in the range of 10 to 20 cm to avoid the problem of the high pressure drop. The recovery of GAPDH and PGK was 57% and 41%, respectively. The purification factor of GPADH and PGK was 144 and 44, respectively. The economical evaluation indicated that the cost of the process was 2.09$/ku GAPDH, which has some commercial values.

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Key concepts: Phosphoglycerate kinase, Glyceraldehyde 3-phosphate dehydrogenase, Dehydrogenase, Chromatography, Chemistry, Glyceraldehyde, Sepharose, Biochemistry