[Construction of eukaryotic expression vector of CD80-IgG1 Fc fragment fusion protein and its expression in CHO cells].

AIM: To construct an eukaryotic expression vector of CD80-IgG1 Fc, and to express the fusion protein in CHO cells. METHODS: The gene encoding the CD80-IgG1 Fc fusion protein were constructed in eukaryotic expression vector pcDNA3.1(+) by means of T-A cloning and subcloning techniques, then was transfected into CHO cells for stable expression. The expression of the fusion protein was detected by Western blot and ELISA. RESULTS: DNA sequencing and restriction endonuclease digestion analysis indicated that the eukaryotic expression vector CD80-IgG1 Fc/pcDNA3.1(+) was successfully constructed. After the recombinant plasmid was transfected into CHO cells, the stable expression of the fusion protein was demonstrated by Western blot and ELISA. CONCLUSION: The eukaryotic expression vector of CD80-IgG1 Fc/pcDNA3.1(+) was successfully constructed and stably expressed in CHO cells, providing basis for anti-tumor study.