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Somatic Embryogenesis and Plant Regeneration from Cultured Leaf Explants of Zea mays

DEBJANI SINHA RAY, Parthadeb Ghosh

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Abstract

Young leaf segments of Zea mays L. seedlings were cultured on Murashige and Skoog's basal nutrient medium supplemented with 2 mg l−1 2, 4-D and sub-cultured on medium containing 8 mg l−1 2,4-D. Two types of callus tissues appeared—embryogenic and non-embryogenic. The embryogenic callus tissue produced numerous somatic embryos which on transfer to media containing low amounts of 2,4-D or ABA produced plantlets. Callus tissues exhibited embryogenic potential for more than 1 year.

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What this paper is about

Young leaf segments of Zea mays L. seedlings were cultured on Murashige and Skoog's basal nutrient medium supplemented with 2 mg l−1 2, 4-D and sub-cultured on medium containing 8 mg l−1 2,4-D. Two types of callus tissues appeared—embryogenic and non-embryogenic. The embryogenic callus tissue produced numerous somatic embryos which on transfer to media containing low amounts of 2,4-D or ABA produced plantlets. Callus tissues exhibited embryogenic potential for more than 1 year.

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Available abstract

Young leaf segments of Zea mays L. seedlings were cultured on Murashige and Skoog's basal nutrient medium supplemented with 2 mg l−1 2, 4-D and sub-cultured on medium containing 8 mg l−1 2,4-D. Two types of callus tissues appeared—embryogenic and non-embryogenic. The embryogenic callus tissue produced numerous somatic embryos which on transfer to media containing low amounts of 2,4-D or ABA produced plantlets. Callus tissues exhibited embryogenic potential for more than 1 year.

Key concepts: Biology, Somatic embryogenesis, Callus, Explant culture, Zea mays, Botany, Tissue culture, Murashige and Skoog medium

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