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2016•International Educational Scientific Research JournalRequires access

SIMULTANEOUS QUANTIFICATION OF ELLAGIC ACID, GALLIC ACID, CHLOROGENIC ACID AND QUERCETIN IN THE EXTRACT OF TERMINALIA TOMENTOSA PLANT AND ITS FORMULATION USING HPTLC

Deepal D. Ghadge, Vikas V. Vaidya, Saurabh H. Patil

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Abstract

A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of ellagic acid, chlorogenic acid, gallic acid and quercetin in the leaf extract of Terminalia tomentosa and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of Butyl acetate: Formic Acid: Distilled Water 14:5:5 (v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.

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What this paper is about

A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of ellagic acid, chlorogenic acid, gallic acid and quercetin in the leaf extract of Terminalia tomentosa and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of Butyl acetate: Formic Acid: Distilled Water 14:5:5 (v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.

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Available abstract

A simple, precise, accurate and rapid High-Performance Thin Layer Chromatographic method has been developed and validated for the simultaneous estimation of of ellagic acid, chlorogenic acid, gallic acid and quercetin in the leaf extract of Terminalia tomentosa and its Formulation. The stationary phase used was precoated silica gel 60F254.The mobile phase used was a mixture of Butyl acetate: Formic Acid: Distilled Water 14:5:5 (v/v). The detection of spots were carried out at 254 nm. This HPTLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. It can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.

Key concepts: Gallic acid, Ellagic acid, Chlorogenic acid, Chromatography, Chemistry, Quercetin, Silica gel, Formic acid

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SIMULTANEOUS QUANTIFICATION OF ELLAGIC ACID, GALLIC ACID, CHLOROGENIC ACID AND QUERCETIN IN THE EXTRACT OF TERMINALIA TOMENTOSA PLANT AND ITS FORMULATION USING HPTLC — Research Paper | ScholarLens