2003•PubMedRequires access

[The simultaneous isolation of outer hair cells and Deiters' cells from the cochlea of the guinea pig].

Qing Chang, Shusheng Gong, Ming Tang, J. Hescheler

Abstract

OBJECTIVE: To investigate the simultaneous method of isolating outer hair cells and Deiters' cells from the organ of Corti of the guinea-pig cochlea. METHOD: The organ of Corti was dissected from the pigmental guinea pig cochlea, and then treated with papain. RESULT: A large number of living outer hair cells and Deiters' cells were obtained synchronously from the guinea pig. CONCLUSION: The key to success in isolating outer hair cells and Deiters' cells from the cochlea is to know very well the anatomical characteristics of the cochlea and keep the contact the organ of Corti.

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Key concepts: Organ of Corti, Cochlea, Outer hair cells, Guinea pig, Anatomy, Biology, Inner ear, Endocrinology