2007•PubMedRequires access

[Different assays for hingamine in the study of biological fluids].

O Iu Strelova, Vozheva Ab, Slonimskaia Ea, Strelkova Lf

Abstract

The authors present a method of hingamine identification in non-biological substances (tablets, powder, syringes) and biological fluids (blood, urine). Isolation was made with chloroform in pH 10. Identification was conducted with thin-layer chromatography, gas chromatography/mass-spectrometry, high-performance liquid chromatography, spectrophotometry in UV region. The quantity was estimated with spectrophotometry in UV region, high-performance liquid chromatography and high-performance thin-layer chromatography. The results of the three methods are comparable.

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Available abstract

Key concepts: Chromatography, Thin-layer chromatography, Chemistry, Spectrophotometry, Biological fluids, Mass spectrometry, Urine, High-performance liquid chromatography