2009•Guizhou nongye kexueRequires access

Mutagenic Effects of γ-Ray and EMS on Seed Vigor of Tobacco

Shi Yuewei, Xueliang Ren, Yi Wang, Yanxia Sun, Xiaoyong Wu

Abstract

To explore appropriate dose of γ-ray and EMS for mutagenesis of tobacco seeds,330 treatments combined with 10 different doses of γ-ray and 11 different concentrations of EMS were designed,and mutagenic effects of γ-ray and EMS on tobacco seeds of K346,NC82,and Yun 85 were studied.The results showed that γ-ray,EMS,tobacco variety,and their interactions have significant effects(P0.01) on seed vigor index.The sequence of γ-ray radiation sensitivity for tobacco varieties is Yun 85K346NC82,and the appropriate dose of γ-ray is 300~350 Gy.The sequence of EMS mutagenic sensitivity for tobacco varieties is Yun 85K346≈NC82,and the appropriate concentration of EMS is 0.35%.The sequence of mutagenic sensitivity of interaction of γ-ray and EMS for tobacco varieties is Yun 85K346≈NC82,and the injury of γ-ray to seed vigor is larger than of EMS.

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Key concepts: Tobacco leaf, Mutagenesis, Biology, Horticulture, Toxicology, Mutation, Genetics, Gene