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Detection of genes for heat-stable enterotoxin in Escherichia coli by biotinylated ST-DNA probes.

Qingyi Zhu, Li Lq, Lin Wm, Zhijun Zhou, Liu Cj

Abstract

Reference strains of enterotoxigenic Escherichia coli (ETEC), non-enterotoxigenic Escherichia coli (non-ETEC), enteropathogenic Escherichia coli (EPEC), enteroinvasive Escherichia coli (EIEC), and other enteropathogenic bacteria were used to prove the reliability of BIO-ST-DNA probe hybridization. In addition, 417 strains of E. coli isolated from children with diarrheal diseases in Shanxi Children's Hospital were examined for BIO-ST-DNA probe hybridization. In the test, BIO-ST-DNA hybridization was compared with suckling mouse assay in identifying ST-ETEC. The results obtained by both methods showed no significant difference. It was found that identification of ST-ETEC using hybridization is a simple, sensitive and more practical method.

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Key concepts: Enterotoxigenic Escherichia coli, Escherichia coli, Enterotoxin, Microbiology, Enteropathogenic Escherichia coli, DNA–DNA hybridization, Biology, Hybridization probe