Callus induction and Organogenesis in an Indian Box-wood (Gardenia latifolia Ait.)

An efficient method to evaluate the most suitable concentration of growth regulators for callus induction and subsequent organogenesis in Gardenia latifolia has been studied. The best callus induction was found in half cut seeds cultured on MS medium fortified with 2, 4-D (2mg/l) concentration produced white callus. Combination of 2, 4-D (2mg/l) + BAP (0.1mg/l) produced 80% of brownish white colour callus which gave the most effective for callus induction. Internodal callus showed maximum percentage of response (55%) in the MS medium containing NAA (0.1mg/l) + BAP (1.0mg/l) produced yellowish and jelly nodular soft callus. Half cut seed callus showed 55% of response observed in the MS medium containing IAA (0.1mg/l) + BAP (0.5mg/l) which was compact and smooth in nature and not shown any response for shoot formation except appearance of shoot buds.