Localization of Inhibition of Platelet Aggregation by Incubation of Platelet Rich EDTA-Plasma at 37°C

Summary By photometric and microscopic technics investigations have been carried out to determine the nature of the inhibition of platelet aggregation caused by incubating platelet rich EDTA-plasma at 37° C. The results clearly indicate that the inhibition must arise from alterations in the platelets themselves. The appearance of an inhibitory substance in plasma and the release of such a substance from the platelets into the plasma have been excluded. Addition of ADP to the inhibited platelets did not influence the inhibition of platelet aggregation.