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[Induction of Escherichia coli K-12 mutants with an increased efficiency of plasmid transformation].

N A Likhacheva, E S Molchanova, Il'iashenko Bn

Abstract

A new rapid method for plasmid transformation of Escherichia coli K-12 cells has been devised. It consists in application of plasmid pMB9 DNA to the surface of an agar medium with 0.05 M CaCl2 and tetracycline (50 micrograms/ml). The recipient cells treated with nitrosoguanidine were drifted on by sectors on the plates with pMB9 DNA. The method enabled the obtaining of 12 mutants with high efficiency of plasmid transformation.

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Available abstract

Key concepts: Plasmid, Escherichia coli, Transformation (genetics), Mutant, Plasmid preparation, Tetracycline, Microbiology, Transformation efficiency