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[Critical evaluation of quantitative immunoglobulin determination in the calf].

G Lambrecht, K Petzoldt

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Abstract

From 3 calves fed with a single limited amount of colostrum 68 blood serum samples were tested comparatively by single radial immunodiffusion (RID) and by nephelometry for their IgG1-, IgA- and IgM-content. In comparison with nephelometry the commercial available RID kits seemed to be insufficient due to deficient agarose plates, difficulties in diluting, and weak reference sera. The results obtained by RID, therefore, were not comparable with those obtained by nephelometry which should be optimized, too.

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What this paper is about

From 3 calves fed with a single limited amount of colostrum 68 blood serum samples were tested comparatively by single radial immunodiffusion (RID) and by nephelometry for their IgG1-, IgA- and IgM-content. In comparison with nephelometry the commercial available RID kits seemed to be insufficient due to deficient agarose plates, difficulties in diluting, and weak reference sera. The results obtained by RID, therefore, were not comparable with those obtained by nephelometry which should be optimized, too.

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Available abstract

From 3 calves fed with a single limited amount of colostrum 68 blood serum samples were tested comparatively by single radial immunodiffusion (RID) and by nephelometry for their IgG1-, IgA- and IgM-content. In comparison with nephelometry the commercial available RID kits seemed to be insufficient due to deficient agarose plates, difficulties in diluting, and weak reference sera. The results obtained by RID, therefore, were not comparable with those obtained by nephelometry which should be optimized, too.

Key concepts: Nephelometry, Radial immunodiffusion, Colostrum, Immunodiffusion, Agarose, Antibody, Chromatography, Chemistry

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