Colloidal Gold for the Detection of Proteins on Blots and Immunoblots

Immunoblotting techniques, in which electrophoretically separated proteins are transferred to the surface of nitrocellulose or nylon membranes (“Western” blots), are becoming increasingly popular in the analysis of protein systems. Transfer techniques in protein blotting have been covered in this series ( Chapter 20 in vol. 1, and Chapters 28 and 29 in this volume). In this chapter we will describe the use of the colloidal gold marking system for the detection of proteins on blots. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.