[Prokaryotic microbial phylogenetic diversity of "Eryuan Niujie" hot spring in Yunnan province, China].

OBJECTIVE: We analyzed the prokaryotic microbial diversity of Eryuan Niujie hot spring, in Yunnan Province, to enrich our knowledge about thermo-stable microbes. METHOD: We constructed bacterial and archaeal 16S rRNA gene libraries, analyzed the sequences and constructed phylogenetic trees to learn the prokaryotic microbial diversity. RESULT: The majority of the prokaryotic microbes in this hot spring were bacteria, while beta-Proteobacteria was the most abundant, next were Bacteroidetes and Chlorobi; the abundance and diversity of archaea were both less than that of bacteria, including Euryarchaeota and Crenarchaeota, while Euryarchaeota was the most abundant.