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Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro.

Guolin Meng, Yunyu Hu, Pu Qin, Rong Wen Lu, Yang Liu, Jun Wang, Jianping Bai, Xinzhi Xu, Dan Li

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Abstract

OBJECTIVE: To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union. METHODS: rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control. RESULTS: Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells. CONCLUSIONS: BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.

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What this paper is about

OBJECTIVE: To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union. METHODS: rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control. RESULTS: Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells. CONCLUSIONS: BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.

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Available abstract

OBJECTIVE: To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union. METHODS: rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control. RESULTS: Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells. CONCLUSIONS: BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.

Key concepts: Bone morphogenetic protein 2, Transfection, Bone morphogenetic protein, Bone morphogenetic protein 7, Fibroblast, Immunohistochemistry, In vitro, Cell biology

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