2008•PubMedRequires access
[Primry research of separation and culture of adult hepatocytes].
Hongling Liu, Shao-jie Xin, Shaoli You, Yan Qing Hu, Yi-hui Rong, Lin Zhou, Yongping Yang, Zhiwei Li, Zhenwen Liu, Panyong Mao
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Abstract
OBJECTIVE: To explore the separation and culture method of adult hepatocytes. METHODS: The isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37 degrees C in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed. RESULTS: Hepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of hepatocytes was for lidocaine metabolism and urea excretion. CONCLUSION: The adult hepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificial liver.