[Microspectrophometric evaluation of DNA as a means of analysis of human leukocyte in vitro proliferative kinetics].
F Piccotti, L Ballaira
Abstract
F Piccotti, L Ballaira
Abstract
Microspectrophotometric determination of in vitro human leucocyte DNA content, with or without PHA stimulation, is reported. The results obtained are compared with those observed with tritiated thymidine and their parallel pattern makes in clear that DNA microspectrophotometry can be employed in the study of proliferative kinetics.
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Microspectrophotometric determination of in vitro human leucocyte DNA content, with or without PHA stimulation, is reported. The results obtained are compared with those observed with tritiated thymidine and their parallel pattern makes in clear that DNA microspectrophotometry can be employed in the study of proliferative kinetics.
Key concepts: Thymidine, In vitro, Kinetics, DNA, DNA synthesis, Stimulation, Molecular biology, Chemistry