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[The study of regulation of connective tissue growth factor gene promoter by transforming growth factor beta1 in pancreatic stellate cells].

Yunwei Sun, Yongping Zhang, Min-Min Qiao, Hua Fu, Yaozong Yuan

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Abstract

OBJECTIVE: To investigate the molecular mechanism for transforming growth factor beta(1) (TGF-beta(1)) on regulation of connective tissue growth factor (CTGF) gene promoter in pancreatic stellate cells (PSCs). METHODS: We tried to transfect the passaged 2 approximately 5 PSCs with pCTGF-luc plasmids, which were composed of CTGF promoter and PGL3 vector. And different concentrations of TGF-beta(1) or stimulation time were used to observe the reaction of luciferase activities by the measurement of dual-luciferase assay system. RESULTS: TGF-beta(1) could enhance the activities of pCTGF-luc in PSCs by means of time and dose dependently. TGF-beta(1) could stimulate the expression of CTGF gene promoter at the high level in a short time. CONCLUSION: TGF-beta(1) could enhance the activities of CTGF promoter in PSCs.

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What this paper is about

OBJECTIVE: To investigate the molecular mechanism for transforming growth factor beta(1) (TGF-beta(1)) on regulation of connective tissue growth factor (CTGF) gene promoter in pancreatic stellate cells (PSCs). METHODS: We tried to transfect the passaged 2 approximately 5 PSCs with pCTGF-luc plasmids, which were composed of CTGF promoter and PGL3 vector. And different concentrations of TGF-beta(1) or stimulation time were used to observe the reaction of luciferase activities by the measurement of dual-luciferase assay system. RESULTS: TGF-beta(1) could enhance the activities of pCTGF-luc in PSCs by means of time and dose dependently. TGF-beta(1) could stimulate the expression of CTGF gene promoter at the high level in a short time. CONCLUSION: TGF-beta(1) could enhance the activities of CTGF promoter in PSCs.

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Available abstract

OBJECTIVE: To investigate the molecular mechanism for transforming growth factor beta(1) (TGF-beta(1)) on regulation of connective tissue growth factor (CTGF) gene promoter in pancreatic stellate cells (PSCs). METHODS: We tried to transfect the passaged 2 approximately 5 PSCs with pCTGF-luc plasmids, which were composed of CTGF promoter and PGL3 vector. And different concentrations of TGF-beta(1) or stimulation time were used to observe the reaction of luciferase activities by the measurement of dual-luciferase assay system. RESULTS: TGF-beta(1) could enhance the activities of pCTGF-luc in PSCs by means of time and dose dependently. TGF-beta(1) could stimulate the expression of CTGF gene promoter at the high level in a short time. CONCLUSION: TGF-beta(1) could enhance the activities of CTGF promoter in PSCs.

Key concepts: CTGF, Growth factor, Luciferase, Transforming growth factor, Connective tissue, Transfection, Hepatic stellate cell, Transforming growth factor beta

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[The study of regulation of connective tissue growth factor gene promoter by transforming growth factor beta1 in pancreatic stellate cells]. — Research Paper | ScholarLens