[Practical use of the Multistat III centrifuge analyzer in an improved method for the determination of the activity of aspartate aminotransferase and alanine aminotransferase].
Graziano Ferrari, Erba Pn
Abstract
Graziano Ferrari, Erba Pn
Abstract
We applied three procedures for measuring aspartate aminotransferase and alanine aminotransferase activities to the Multistat III MCA. Assay conditions were almost exactly as prescribed by the Scandinavian Committee on Enzymes and the International Federation of Clinical Chemistry. Owing to the overall good performance of the analyzer and its loader module, results of 20 samples will be available in 10 minutes, including preincubation with pyridoxal phosphate. Details of procedures are given; there are no differences in activities of both aminotransferases determined with three methods, allowing the user to take a choice according to personal preference.
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We applied three procedures for measuring aspartate aminotransferase and alanine aminotransferase activities to the Multistat III MCA. Assay conditions were almost exactly as prescribed by the Scandinavian Committee on Enzymes and the International Federation of Clinical Chemistry. Owing to the overall good performance of the analyzer and its loader module, results of 20 samples will be available in 10 minutes, including preincubation with pyridoxal phosphate. Details of procedures are given; there are no differences in activities of both aminotransferases determined with three methods, allowing the user to take a choice according to personal preference.
Key concepts: Alanine aminotransferase, Aspartate Aminotransferases, Spectrum analyzer, Alanine transaminase, Chemistry, Centrifuge, Alanine, Pyridoxal phosphate