ScholarLens Back to search
1995•PubMedRequires access

Detection of Bacillus larvae spores in Argentinian honeys by using a semi-selective medium.

Adriana Mónica Alippi

Open publisher page 46 citations

Abstract

A semi-selective medium for the detection in Argentinian honeys of spores of Bacillus larvae, a pathogen of American foulbrood, was developed. The technique involves dilution of samples (1:2) in phosphate buffer, concentration of spores by centrifugation and heat treatment prior to inoculation. Two media (JNxPa and JNxPb) were prepared from J-agar, to which nalidixic acid and pipemidic acid were added. Both JNxP media were reliable for the isolation of B. larvae colonies and, at the same time, prevented the development of other Bacillus species which normally develop on the plates before B. larvae spores can germinate.

About this research paper

What this paper is about

A semi-selective medium for the detection in Argentinian honeys of spores of Bacillus larvae, a pathogen of American foulbrood, was developed. The technique involves dilution of samples (1:2) in phosphate buffer, concentration of spores by centrifugation and heat treatment prior to inoculation. Two media (JNxPa and JNxPb) were prepared from J-agar, to which nalidixic acid and pipemidic acid were added. Both JNxP media were reliable for the isolation of B. larvae colonies and, at the same time, prevented the development of other Bacillus species which normally develop on the plates before B. larvae spores can germinate.

Why it matters

OpenAlex reports 46 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A semi-selective medium for the detection in Argentinian honeys of spores of Bacillus larvae, a pathogen of American foulbrood, was developed. The technique involves dilution of samples (1:2) in phosphate buffer, concentration of spores by centrifugation and heat treatment prior to inoculation. Two media (JNxPa and JNxPb) were prepared from J-agar, to which nalidixic acid and pipemidic acid were added. Both JNxP media were reliable for the isolation of B. larvae colonies and, at the same time, prevented the development of other Bacillus species which normally develop on the plates before B. larvae spores can germinate.

Key concepts: Spore, Nalidixic acid, American foulbrood, Agar, Microbiology, Germination, Biology, Isolation (microbiology)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Detection of Bacillus larvae spores in Argentinian honeys by using a semi-selective medium. — Research Paper | ScholarLens