Primer Extension Methods for Determination of β1-Adrenergic Receptor mRNA Start Sites

Primer extension is often used to map the 5′ end of RNA ( 1 ). A single-stranded, end-labeled DNA primer is hybridized to RNA first. Using an RNA-dependent DNA polymerase (reverse transcriptase [RT]) and nonradioactive deoxynucleotides, the primer is extended to yield cDNA. The cDNA is then analyzed on a sequencing gel to determine nucleotide length. The length of the cDNA reflects the distance between the primer and the 5′ end of RNA, and hence, maps the transcription start sites. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.