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[Expression of bFGF in primary human lens epithelial cells].

D Liu, Jianli Wu

Abstract

OBJECTIVE: To study the mechanism of after cataract formation and investigate the expression of basic fibroblast growth factor (bFGF) in primary human lens epithelial cells (PHLECs). METHODS: Human lens epithelial cells were cultured in vitro, and the expression of bFGF was detected with immunohistochemistry and in situ hybridization. RESULTS: PHLECs express bFGF in protein and mRNA levels. CONCLUSION: Based on bFGF promoting PHLEC growth and differentiation, the results suggest that bFGF from PHLECs take part in promoting such cell growth and differentiation.

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Key concepts: Basic fibroblast growth factor, In situ hybridization, In vitro, Cell biology, Immunohistochemistry, Lens (geology), Biology, Messenger RNA