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A ciliary neurotrophic factor-sensitive human myeloma cell line.

Z J Gu, X G Zhang, M M Hallet, Zhao‐Yang Lu, John Wijdenes, J.F. Rossi, Bernard Klein

Abstract

We obtained a human myeloma cell line (XG4-CNTF) whose growth was completely dependent on addition of ciliary neurotrophic factor (CNTF). Half-maximal proliferation was induced by adding 20 pg/mL CNTF. Response to CNTF correlated with expression of membrane CNTF receptor alpha-chain (CNTFR alpha), as shown by PCR analysis and immunostaining with anti-CNTFR alpha antibodies. CNTF-induced proliferation was completely inhibited by antibodies to gp130 interleukin-6 (IL-6) transducer, unlike antibodies to IL-6 or IL-6 receptor (IL-6R). Growth of XG4-CNTF cells using the gp130 IL-6 transducer was also supported by other cytokines: IL-6, leukemia inhibitory factor (LIF), and oncostatin M (OM). This cell line should be very useful for studying the interactions of IL-6-related cytokines with their receptors.

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Key concepts: Ciliary neurotrophic factor, Oncostatin M, Glycoprotein 130, Leukemia inhibitory factor, Leukemia inhibitory factor receptor, Interleukin 6, Cell culture, Interleukin-6 receptor