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2011•Methods in molecular biologyRequires access

AFM to Study Bio/Nonbio Interactions

Holger Schönherr

Open publisher page 2 citations

Abstract

This chapter describes a versatile approach to immobilize proteins and other biomolecules on reactive self-assembled monolayers on gold as a means to study interactions (forces) between these biomolecules and nonbiological entities. Biomolecules are either immobilized on the surface of flat substrates or on the surface of gold-coated atomic force microscopy (AFM) probe tips. In addition to the immobilization protocols, the actual AFM experiments in liquid and the quantitative analysis of adhesive forces receive attention. With these procedures and tools at hand, a wide variety of problems in the area of bio/nonbio interactions can be addressed experimentally.

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What this paper is about

This chapter describes a versatile approach to immobilize proteins and other biomolecules on reactive self-assembled monolayers on gold as a means to study interactions (forces) between these biomolecules and nonbiological entities. Biomolecules are either immobilized on the surface of flat substrates or on the surface of gold-coated atomic force microscopy (AFM) probe tips. In addition to the immobilization protocols, the actual AFM experiments in liquid and the quantitative analysis of adhesive forces receive attention. With these procedures and tools at hand, a wide variety of problems in the area of bio/nonbio interactions can be addressed experimentally.

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OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

This chapter describes a versatile approach to immobilize proteins and other biomolecules on reactive self-assembled monolayers on gold as a means to study interactions (forces) between these biomolecules and nonbiological entities. Biomolecules are either immobilized on the surface of flat substrates or on the surface of gold-coated atomic force microscopy (AFM) probe tips. In addition to the immobilization protocols, the actual AFM experiments in liquid and the quantitative analysis of adhesive forces receive attention. With these procedures and tools at hand, a wide variety of problems in the area of bio/nonbio interactions can be addressed experimentally.

Key concepts: Biomolecule, Atomic force microscopy, Nanotechnology, Monolayer, Materials science, Chemistry, Adhesive, Layer (electronics)

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