2006•PubMedRequires access
[Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots].
Shihai Yang, Xiaofeng Liu, Xin Shen, Jun-hua Zheng
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Abstract
OBJECTIVE: To study the induction of hairy roots of Glycyrrhiza uralensis and effect of some physical as well as chemical factors on the growth of hairy roots. METHOD: Hypocotyls and cotyledons of G. uralensis were infected with Agrobacterium rhizogenes LBA9402 and A. rhizogenes R1601, respectively. RESULT: Hairy roots were induced by two strains of A. rhizogenes, and LBA9402 showed astronger infective ability. The frequency of transformation of hypocotyls was higher than that of cotyledons. Among the different media tested, WP medium yielded the fastest growth rate of hairy roots. Light inhibited the growth of hairy roots. The total amout of 5 flavonoids in hairy roots was 1.5 times as much as that in the callus, among them licochalcone content was 15.5 times as that of callus. CONCLUSION: The in vitro culture system of the hairy roots established laid a foundation for mass production of flavonoids by hairy root culture.