2001•PubMedRequires access
[Pilot study on mutations of p53 gene in laryngeal carcinoma].
Guoying Zhou, X Zhang, Lui S, Dongxin Lin, Chen Liang, Xiaobo Yang
Open publisher page 1 citations
Abstract
OBJECTIVE: To determine the characteristic and frequency of the point mutation of p53 gene. METHODS: 22 cases of laryngeal squamous cell carcinoma (LSCC) with positive expression of mutant p53 protein and 2 cases of normal laryngeal epithelium as control were examined by means of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), single strand conformation polymorphism (SSCP) and DNA sequencing analyses. RESULTS: 3 cases of LSCC had the point mutation on codons 248, 250 and 254 of exon 7 in p53 gene. None of the cases had the point mutation on codon 249. CONCLUSION: These findings suggest that the point mutation on codon 249 of exon 7 in p53 gene may be uncommon in LSCC. SSCP and DNA sequencing analyses are sensitive and rapid methods for detecting p53 gene mutation.