Comparison of two assays for the detection of haemolysins of Aeromonas species.
Jamuna Vadivelu, Puthucheary Sd, Prakash Navaratnam
Abstract
Jamuna Vadivelu, Puthucheary Sd, Prakash Navaratnam
Abstract
The haemolysins produced by Aeromonas species were detected and compared by two assay methods--a modified blood agar plate assay and the rabbit erythrocyte haemolysin method. Both assays showed a high level of agreement (86%). The titres of the rabbit erythrocyte haemolysin assay correlated with the haemolytic zone diameter of the ox blood agar assay. In addition the agar haemolysin assay had simple media requirements, was easy to perform and results were well defined.
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The haemolysins produced by Aeromonas species were detected and compared by two assay methods--a modified blood agar plate assay and the rabbit erythrocyte haemolysin method. Both assays showed a high level of agreement (86%). The titres of the rabbit erythrocyte haemolysin assay correlated with the haemolytic zone diameter of the ox blood agar assay. In addition the agar haemolysin assay had simple media requirements, was easy to perform and results were well defined.
Key concepts: Hemolysin, Aeromonas, Agar plate, Hemolysis, Microbiology, Agar, Medicine, Bacteria