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Umbilical cord blood as a source of CD34 positive haematopoietic stem cells.

Rao Sg, Desai Ss, AP Goud, Anupama Deora, Amin Mk, Gangal Sg

Abstract

In this paper we have used a monoclonal antibody to CD34 an antigen expressed solely on stem cells, and stem cell colony assays to show that umbilical cord blood has nearly the same number of functional stem cells as compared to normal bone-marrow. The number of CD34+ve cells in cord blood being 2 to 2.7 per cent, whereas bone-marrow had 3 to 3.5 per cent. The multi-potent colony forming cells (CFU-GEMM) were 60 +/- 18 in cord blood per 2 x 10(5) mononuclear cells (MNCs), whereas normal bone-marrow had 70 +/- 10 per 2 x 10(5) MNCs. Enrichment of these stem cells on Percoll gradients was successful for normal bone-marrow but not for cord blood.

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Key concepts: Bone marrow, Haematopoiesis, Stem cell, CD34, Cord blood, Umbilical cord, Percoll, Peripheral blood mononuclear cell