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[Expression of human granulocyte-macrophage colony stimulating factor(hGM-CSF) by recombinant vaccinia virus and its effect on immunogenicity].
H Liu, Z Zhang, Lan Yao
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Abstract
hGM-CSF was inserted into the down stream of P11K promoter of pJSB1175, which consists of HSV-1gD under the promoter P7.5K. The constructed plasmid pJSB1175D/GM-CSF was cotransfected with vaccinia virus Tiantan Strain into TK-143. After selection and screening, recombinant virus RVJSB11D/GM-CSF was harvested. It was proved that the recombinant virus could express GM-CSF and HSV-1gD at the same time by MTT assay and IFA in vitro. In vivo study showed that the expression of GM-CSF by RVJSB1175D/GM-CSF did not interfere with the HSV-1gD antibody production.