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[In vitro expansion of human umbilical cord blood hematopiotic cells and its subpopulation].

Cao Xs, Zongshu Zou, Yu Wang

Abstract

OBJECTIVE: To study the effect of expansion of cord blood stem cells in vitro. METHODS: The mononuclear cells of human umbilical cord blood was cultured in vitro and the cell cycle and CD34+ subset analysis was performed on these cell preparations using flow cytometry. RESULTS: After 35 days, the number of nucleated cells showed over a comulative 252 fold expansion. CD34+ cells increased 50-fold in 7-21 days. CD33+ cells increased 280-fold during 7-28 days. CD19+ and CD34+ cells disappared after 14 days. The cells remained in G0/G1 phase and increased in the S phase. CONCLUSION: Single umbilical cord blood would be enough to transplant to adults if suitable time and a novel expansion strategies are set.

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Key concepts: Umbilical cord, CD34, Cord blood, Peripheral blood mononuclear cell, Flow cytometry, Andrology, In vitro, CD19