[Calcofluor-white staining in the identification of Pneumocystis carinii].
Franz J. Allerberger, Prior C, Dietger Niederwieser, Dierich Mp
Abstract
Franz J. Allerberger, Prior C, Dietger Niederwieser, Dierich Mp
Abstract
Calcofluor white stain is widely used as a fluorescent stain in mycology. Recently, it was found that by calcofluor white staining Pneumocystis carinii cysts exert a highly characteristic staining pattern. We retested fifteen clinical specimens which were known to be positive for Pneumocystis carinii (diagnosis made by a methenamine silver stain). Calcofluor white stain proved to be a simple, rapid, and inexpensive method for detecting Pneumocystis carinii in clinical specimens.
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Calcofluor white stain is widely used as a fluorescent stain in mycology. Recently, it was found that by calcofluor white staining Pneumocystis carinii cysts exert a highly characteristic staining pattern. We retested fifteen clinical specimens which were known to be positive for Pneumocystis carinii (diagnosis made by a methenamine silver stain). Calcofluor white stain proved to be a simple, rapid, and inexpensive method for detecting Pneumocystis carinii in clinical specimens.
Key concepts: Pneumocystis carinii, Stain, Staining, Grocott's methenamine silver stain, Silver stain, Pathology, Microbiology, Biology