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Rehydratable agarose gels: use of low EEO agarose without charged additives.

Erich Gombocz, Andreas Chrambach

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Abstract

Agarose [SeaKem HGT(P)] gels of 0.5% to 1.2% can be dried at room temperature and rehydrated to 84-85% of their original water content. Mobilities, using the 84-85% rehydrated gels, are indistinguishable from those on the original gels. This suggests that the fiber structure and effective pore size of agarose are independent on the 16% loosely bound (and probably synersed) water.

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What this paper is about

Agarose [SeaKem HGT(P)] gels of 0.5% to 1.2% can be dried at room temperature and rehydrated to 84-85% of their original water content. Mobilities, using the 84-85% rehydrated gels, are indistinguishable from those on the original gels. This suggests that the fiber structure and effective pore size of agarose are independent on the 16% loosely bound (and probably synersed) water.

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Available abstract

Agarose [SeaKem HGT(P)] gels of 0.5% to 1.2% can be dried at room temperature and rehydrated to 84-85% of their original water content. Mobilities, using the 84-85% rehydrated gels, are indistinguishable from those on the original gels. This suggests that the fiber structure and effective pore size of agarose are independent on the 16% loosely bound (and probably synersed) water.

Key concepts: Agarose, Chemistry, Bound water, Chromatography, Chemical engineering, Biophysics, Organic chemistry, Molecule

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