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alpha-Anordrin-induced apoptosis of leukemia K562 cells is not prevented by cicloheximide.

Liguang Lou, Bin Xu

Abstract

AIM: To study effect of protein synthesis inhibitor cicloheximide (Cic) on the apoptosis induced by alpha-anordrin (Ano) in leukemia K562 cells. METHODS: Morphological changes were observed by fluorescent microscopy. DNA content was measured by flow cytometry. DNA fragmentation was analyzed by agarose gel electrophoresis. RESULTS: Exposure of K562 cells to Ano 50 mumol.L-1 for 24 h induced apoptotic cell death. Cic 1 mumol.L-1 did not abrogate or delay this effect. Indeed, Ano-induced apoptosis was augmented by Cic. Cic 100 mumol.L-1 itself stimulated 25% K562 cell apoptosis after 24-h culture. CONCLUSION: Ano-induced apoptosis was independent of de novo protein synthesis.

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Key concepts: Apoptosis, K562 cells, Agarose gel electrophoresis, DNA fragmentation, Molecular biology, Flow cytometry, Leukemia, Biology