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2013•Methods in molecular biologyRequires access

Analysis of Gene Expression in Skin Using Laser Capture Microdissection

Briana Lee, Mikhail Geyfman, Bogi Andersen, Xing Dai

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Abstract

Gene expression analysis is a useful tool to study the molecular mechanisms underlying skin development and homeostasis. Here we describe a method that utilizes laser capture microdissection (LCM) to isolate RNAs from localized areas of skin, allowing the characterization of gene expression by RT-PCR and microarray technologies.

About this research paper

What this paper is about

Gene expression analysis is a useful tool to study the molecular mechanisms underlying skin development and homeostasis. Here we describe a method that utilizes laser capture microdissection (LCM) to isolate RNAs from localized areas of skin, allowing the characterization of gene expression by RT-PCR and microarray technologies.

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OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Gene expression analysis is a useful tool to study the molecular mechanisms underlying skin development and homeostasis. Here we describe a method that utilizes laser capture microdissection (LCM) to isolate RNAs from localized areas of skin, allowing the characterization of gene expression by RT-PCR and microarray technologies.

Key concepts: Laser capture microdissection, Microdissection, Gene expression, Microarray, Biology, Gene, Microarray analysis techniques, Computational biology

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