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gyrA gene mutations in quinolone-resistant Shigellae flexneri.

Qian Xiang, Shouyi Yu, Hong Wang

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Abstract

OBJECTIVE: To study the resistance mechanism of quinolone against Shigellae flexneri. METHODS: The N-terminal coding region of gyrA gene of 38 quinolone-resistant Shigellae flexneri were amplified by PCR and mutations detected by single- strand conformation polymorphism (SSCP), followed by sequence analysis. RESULTS: Eight isolates were found to be positive for gyrA mutations, and DNA sequence analysis of gyrA gene revealed 2 mutations that resulted in changes of the amino acids (Ser-83--Leu, Asp-87--Gly). CONCLUSION: gryA gene mutations is closely related to high resistance of Shigellae flexneri to quinolone.

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What this paper is about

OBJECTIVE: To study the resistance mechanism of quinolone against Shigellae flexneri. METHODS: The N-terminal coding region of gyrA gene of 38 quinolone-resistant Shigellae flexneri were amplified by PCR and mutations detected by single- strand conformation polymorphism (SSCP), followed by sequence analysis. RESULTS: Eight isolates were found to be positive for gyrA mutations, and DNA sequence analysis of gyrA gene revealed 2 mutations that resulted in changes of the amino acids (Ser-83--Leu, Asp-87--Gly). CONCLUSION: gryA gene mutations is closely related to high resistance of Shigellae flexneri to quinolone.

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Available abstract

OBJECTIVE: To study the resistance mechanism of quinolone against Shigellae flexneri. METHODS: The N-terminal coding region of gyrA gene of 38 quinolone-resistant Shigellae flexneri were amplified by PCR and mutations detected by single- strand conformation polymorphism (SSCP), followed by sequence analysis. RESULTS: Eight isolates were found to be positive for gyrA mutations, and DNA sequence analysis of gyrA gene revealed 2 mutations that resulted in changes of the amino acids (Ser-83--Leu, Asp-87--Gly). CONCLUSION: gryA gene mutations is closely related to high resistance of Shigellae flexneri to quinolone.

Key concepts: Quinolone, Single-strand conformation polymorphism, Gene, Genetics, Biology, Shigella flexneri, Coding region, Sequence analysis

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