[Cyanide insensitive respiration of Candida lipolytica yeasts].

The effect of rotenone, antimycin A, oligomycin, 2,4,-DNP, and various subtrates on the appearance of cyanide-resistant respiration of Candida lipolytica yeast was studied. Rotenone inhibited the development of cyanide-resistant respiration, but did not prevent its origination. Antimycin A and oligomycin increased the rate of the appearance of cyanide-resistant respiration. If the resting yeast culture was incubated with 2,4-DNP, glucose, or glycerol, the development of the alternative pathway of electron transport was inhibited. Such substrates as alpha-glycerophosphate, citrate, and D,L-lactate had no effect on the time of appearance of cyanide-resistant respiration. Therefore, in the stationary growth phase, an alternative, cyanide-resistant pathway of electron transport results from the inhibition of the rate of electron transport in the respiration chain.