1990•PubMedRequires access
[The development of a radioimmunological method for determining autoantibodies to thyroglobulin].
A I Shcherban', Cherkesova Ts, Bogdanovich Af, V I Vasilevskiĭ, Eugeni P. Demidchik, Minaĭlo Ti
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Abstract
A radioimmunoassay to determine a titer of autoantibodies to thyroglobulin in human blood serum was developed. The scheme of analysis included a reaction between 125I-thyroglobulin and autoantibodies to thyroglobulin with subsequent separation of the produced immune complex and free polyethylene glycol-labeled thyroglobulin. A high specificity of the radioimmunoassay is ensured by the use of highly purified thyroglobulin for obtaining the labeled agent. A possibility of the use of the method for the diagnosis of autoimmune thyroid diseases was shown.