[Effects of fluorescent labeling on cytotoxic characteristic and cell distribution of nanopolystyrene].

OBJECTIVE: To explore the feasibility that studied with fluorescent labeling nano-scale materials cell kinetics. METHODS: At the doses of 500, 250, 125, 63, 32 and 16 microg/ml and at the times of 48, 24, 16, 8, 4 and 1 h , MTT assay was applied to observe the inhibitory effects in BEAS-2B and THP-1 cell lines treated with fluorescent labeling nanopolystyrene and non-labeling nanopolystyrene. Confocal microscopy and transmission electron microscopy were applied to image the translocation of fluorescent labeling nanopolystyrene and non-labeling nano polystyrene into cells. Results The tendency of cell inhibitory rate both in fluorescent labeling nanopolystyrene and non-labeling nanopolystyrene was same, which increase with the dose increase. There were no significant differences in the same dose group for two kinds of materials (P > 0.05). The two kinds of materials were found to enter the BEAS-2B and THP-1 on 1 h, and localized within the cell nucleus on 4 h. CONCLUSION: There were no obvious influence on cytotoxicity and cell distribution for fluorescent labeling . Fluorescent labeling nanopolystyrene could apply to study cell kinetics equal to the same size nanopolystyrene.