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Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells.

Hideaki Yasuhara, Seiji Sonobe, Hiroh Shibaoka

Open publisher page 65 citations

Abstract

Treatment with 20 microM brefeldin A (BFA) for 60 min caused the disassembly of the Golgi apparatus in tobacco BY-2 cells, and the effect of BFA was reversible. Connections between Golgi cisternae and the ER were observed in cells that had been treated for 15 min with BFA. BFA applied to cells at metaphase allowed the cells to form aniline blue-positive cell plates but not to complete cytokinesis. BFA seems to inhibit cytokinesis by shutting off the supply of cell-plate materials by disassembling the Golgi apparatus.

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What this paper is about

Treatment with 20 microM brefeldin A (BFA) for 60 min caused the disassembly of the Golgi apparatus in tobacco BY-2 cells, and the effect of BFA was reversible. Connections between Golgi cisternae and the ER were observed in cells that had been treated for 15 min with BFA. BFA applied to cells at metaphase allowed the cells to form aniline blue-positive cell plates but not to complete cytokinesis. BFA seems to inhibit cytokinesis by shutting off the supply of cell-plate materials by disassembling the Golgi apparatus.

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OpenAlex reports 65 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Treatment with 20 microM brefeldin A (BFA) for 60 min caused the disassembly of the Golgi apparatus in tobacco BY-2 cells, and the effect of BFA was reversible. Connections between Golgi cisternae and the ER were observed in cells that had been treated for 15 min with BFA. BFA applied to cells at metaphase allowed the cells to form aniline blue-positive cell plates but not to complete cytokinesis. BFA seems to inhibit cytokinesis by shutting off the supply of cell-plate materials by disassembling the Golgi apparatus.

Key concepts: Cell plate, Brefeldin A, Golgi apparatus, Cytokinesis, Cell biology, Chemistry, Phragmoplast, Cell

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Effects of brefeldin A on the formation of the cell plate in tobacco BY-2 cells. — Research Paper | ScholarLens